Arrangement for determining the concentration of nucleic acids

摘要

An arrangement for determining the concentration of nucleic acids in a sample in a microfluidic device. In at least one embodiment, the arrangement having a) a first chamber into which a the sample is introduced, b) an element in the first chamber for carrying out a number of cycles of an amplification reaction to be carried out in cycles for amplifying nucleic acids, c) a second chamber, connectable to the first chamber by way of fluidic communication, equipped with an element to determine concentrations of amplified nucleic acids, d) a control unit to introduce a defined volume of a solution containing reagents for the amplification reaction into the first chamber and to transfer a defined volume from said first chamber to the second chamber.

主权项

1. An arrangement, comprising:
a microfluidic device including,
a first chamber with an element for amplifying nucleic acids by means of an amplification reaction to be carried out in cycles,a second chamber, connectable to the first chamber by way of fluidic communication, equipped with an element to determine concentrations of amplified nucleic acids, andat least one valve for interrupting and/or releasing fluidic communication between the chambers; a single pump to transfer nucleic acids; and a control unit to control the at least one valve and the pump, and to introduce a defined volume of a solution containing reagents for the amplification reaction into the first chamber and to transfer a defined volume from said first chamber to the second chamber, wherein said control unit is connected to the second chamber, said pump and said at least one valve and being responsive to a signal transmitted from said element of the second chamber to said control unit for operating said pump and/or said at least one valve based on the concentration of amplified nucleic acids determined by said element.