Over-expression of a putative oxidoreductase (<i>UcpA</i>) for increasing furfural or 5-hydroxymethylfurfural tolerance

摘要

The subject invention pertains to overexpression of a putative oxidoreductase (ucpA) for increasing furfural tolerance in genetically modified microorganisms. Genetically modified microorganisms capable of overexpressing UcpA are also provided. Increased expression of ucpA was shown to increase furfural tolerance by 50%, and to permit the fermentation of sugars to products in the presence of 15 mM furfural.

主权项

1. An isolated bacterial, fungal or yeast cell having increased UcpA activity as compared to a reference bacterial, fungal or yeast cell, wherein said bacterial, fungal or yeast cell having increased UcpA activity exhibits increased tolerance to furfural and/or 5-hydroxymethylfurfural (5 -HMF), wherein said bacterial, fungal or yeast cell expresses increased amounts of a UcpA polypeptide comprising SEQ ID NO: 1.