发明名称 |
METHOD FOR POSITIONING AND INTEGRATING TRANSGENE AND USE THEREOF |
摘要 |
Provided in the present invention is a method for positioning and integrating transgene and a use thereof. Specifically provided is a variant loxP element. The sequence “ATAAT” of an reverse repeated sequence in a wild type loxp locus is mutated into “CACCT”, i.e., a variant loxp element. Also provided in the present invention are a construct comprising the variant loxP element, a vector or host cell comprising the construct and a method for preparing a transgenic animal using the vector and the host cell. |
申请公布号 |
US2016304906(A1) |
申请公布日期 |
2016.10.20 |
申请号 |
US201315024405 |
申请日期 |
2013.09.28 |
申请人 |
SHANGHAI GENON BIOENGINEERING CO., LTD ;SHANGHAI TRANSGENIC RESEARCH CENTER |
发明人 |
CHENG Guoxiang;CHEN Jianquan;LIU Siguo |
分类号 |
C12N15/90;A01K67/027 |
主分类号 |
C12N15/90 |
代理机构 |
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代理人 |
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主权项 |
1. A mutant loxP element comprising the sequence as shown in SEQ ID NO.: 30. |
地址 |
Shnaghai CN |