MESENCHYMAL CELL-CONTAINING ERYTHROCYTE FRACTION, METHOD FOR SEPARATING MESENCHYMAL CELL AND METHOD FOR PRODUCING TRANSPLANTATION TISSUE

摘要

PROBLEM TO BE SOLVED: To separate mesenchymal cells not containing hematopoietic system stem cells and inflammatory leukocytes from a bone marrow fluid with safe clinical grade reagents.

SOLUTION: A bone marrow fluid is mixed with an erythrocyte-precipitating agent to separate a mesenchymal cell-containing erythrocyte fraction. The mesenchymal cell-containing erythrocyte fraction, wherein the concentration of nucleated cells except the mesenchymal cells is reduced to 1/2 to 1/100 of that of the non-separated bone marrow fluid.

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