抗ＴＮＦ薬及び自己抗体の検出用アッセイ

摘要

PROBLEM TO BE SOLVED: To provide a method for determining the presence or level of an autoantibody to a therapeutic antibody for treating an autoimmune disease in a sample.SOLUTION: A method comprises; (a) bringing a labeled therapeutic antibody into contact with the sample to form a labeled complex with the autoantibody; (b) subjecting the labeled complex to size exclusion chromatography to separate the labeled complex from a free labeled therapeutic antibody and to detect an amount of the labeled complex and an amount of the free labeled therapeutic antibody; and (c) comparing the amount of the labeled complex and the amount of the free labeled therapeutic antibody detected in the step (b) with a standard curve of known amounts of the autoantibody to determine the presence or level of the autoantibody.SELECTED DRAWING: Figure 4