TISSUE SCAFFOLDS FOR ELECTRICALLY EXCITABLE CELLS

摘要

Inventive concepts relate generally to nanofibrous scaffolds useful for electrophysiological assays. Scaffolds include polymeric nanofibrous components and electrically excitable cells immobilized at a distinct cell seeding domains on the scaffold. Methods and kits including the scaffolds are also described.

主权项

1. A nanofibrous scaffold comprising:
(a) a layer of unaligned polymeric nanofibers; (b) a first population of electrically excitable cells immobilized at a first cell seeding domain on the scaffold; (c) a second population of electrically excitable cells immobilized at a second cell seeding domain on the scaffold at a site that is distinct from the first cell seeding domain; and (d) at least first and second aligned nanofibrous conduits provided on a surface of the unaligned nanofibers, wherein the first conduit is in contact with the first cell seeding domain, and the second conduit is in contact with the second cell seeding domain, and wherein the first conduit and second conduit contact each other at an intersection.