SEMI-SUBMERSIBLE MICROSCOPE OBJECTIVE WITH PROTECTIVE ELEMENT AND USE OF THE SAME IN MULTIPHOTON IMAGING METHOD

摘要

A semi-submersible microscope objective (100) includes a microscope objective having a protective barrel (120) with an optical inlet (122) and optical outlet (124), and a protective element (130) affixed to the microscope objective, sealing the optical outlet (124) but not the optical inlet (122). A transparent portion (132) of the protective element is aligned with the optical exit (124). The protective element is separable from the microscope objective without damaging the microscope objective. Use of the semi-submersible microscope objective in a multiphoton imaging method is also disclosed.