PARTICLE BASED IMMUNOASSAY WITH ALTERNATING CURRENT ELECTROKINETICS

摘要

Disclosed are methods, devices and systems of an immunoassay using an alternate current electrokinetic platform. Also disclosed are methods of separating and detecting analytes from a sample using the disclosed methods.

主权项

1. An immunoassay device for detecting an analyte in a sample, the device comprising:
(a) a microelectrode array, the array capable of establishing an AC electrokinetic field region and isolating a bead complex in a high conductivity buffer, wherein the bead complex comprises a functionalized bead bound to a labelled-primary antibody; (b) a fluidic cartridge, the fluidic cartridge capable of housing the microelectrode array and further comprising at least one port for addition and removal of buffers and reagents; and (c) a fluorescent or luminescent detection system, whereby the presence, absence and/or amount of said analyte in the sample is determined by assessing fluorescence or luminescence from the isolated bead complex bound to a secondary antibody labelled with a fluorescent or luminescent probe.