摘要 |
<p>The present invention provides methods and compositions relating to the labeling of target cells with nanometer scale fluorescent semiconductors referred to as quantum dots (QDs). Specifically, a delivery system is disclosed based on the use of negatively charged QDs for delivery of a tracking fluorescent signal into the cytosol of target cells via a passive endocytosis-mediated delivery process. In a specific embodiment of the invention the target cell is a stem cell, preferably a mesenchymal stem cell (MSC). Such labeled MSCs provide a means for tracking the distribution and fate of MSCs that have been administered to a subject to promote cardiac repair. The invention is based on the discovery that MSCs can be tracked in vitro for up to at least 6 weeks. Additionally, QDs delivered in vivo can be tracked for up to at least 8 weeks, thereby permitting for the first time, the complete 3-D reconstruction of the locations of all MSCs following administration into a host.</p> |
申请人 |
THE TRUSTEES OF COLUMIBA UNIVERSITY IN THE CITY OF NEW YORK;THE RESEARCH FOUNDATION OF STATE UNIVERSITY OF NEW YORK;COHEN, IRA, S.;ROSEN, AMY, B.;BRINK, PETER, R.;GAUDETTE, GLENN;ROSEN, MICHAEL, R.;ROBINSON, RICHARD, B. |
发明人 |
COHEN, IRA, S.;ROSEN, AMY, B.;BRINK, PETER, R.;GAUDETTE, GLENN;ROSEN, MICHAEL, R.;ROBINSON, RICHARD, B. |