AMORPHOUS INORGANIC POLYPHOSPHATE-CALCIUM-PHOSPHATE AND CARBONATE PARTICLES AS MORPHOGENETICALLY ACTIVE COATINGS AND SCAFFOLDS

摘要

This invention concerns a method for the production of amorphous, nano-or microparticular materials based on inorganic polyphosphate (polyP) and calcium phosphate or calcium carbonate that show osteogenic activity. In one aspect of the invention, the inventor shows that amorphous calcium polyphosphate (Ca-polyP) microparticles can be used for biological functionalization of titanium alloy surfaces. The inventive method allows the fabrication of a durable and stable, almost homogeneous and morphogenetically active Ca-polyP layer on titanium oxidized Ti-6Al-4V scaffolds that supports the growth and enhances the functional activity of bone cells, in contrast to biologically inert non-modified titanium surfaces. A preferred aspect relates to the formation of amorphous calcium phosphate (CaP) particles in the presence of low concentrations of sodium polyP. This material causes a strong upregulation of the expression of proteins involved in bone formation. A further aspect of the invention concerns a material containing polyP-stabilized ACC and small amounts of vaterite that exhibits osteogenic activity and supports the regeneration of the implant region in animal experiments. The amorphous materials according to this invention have the potential to be used for bone implants.