METHODS AND COMPOSITIONS FOR TARGETED GENETIC MODIFICATIONS AND METHODS OF USE
摘要
Methods and compositions are provided for generating targeted genetic modifications on the Y chromosome or a challenging target locus. Compositions include an in vitro culture comprising an XY pluripotent and/or totipotent animal cell (i.e., XY ES cells or XY iPS cells) having a modification that decreases the level and/or activity of an Sry protein; and, culturing these cells in a medium that promotes development of XY F0 fertile females. Such compositions find use in various methods for making a fertile female XY non-human mammal in an F0 generation.
申请公布号
WO2015200805(A4)
申请公布日期
2016.07.14
申请号
WO2015US38001
申请日期
2015.06.26
申请人
REGENERON PHARMACEUTICALS, INC.
发明人
FRENDEWEY, DAVID;DROGUETT, GUSTAVO;GAGLIARDI, ANTHONY;KUNO, JUNKO;AUERBACH, WOJTEK;VALENZUELA, DAVID M.