Breast cancer biomarker signatures for invasiveness and prognosis

摘要

MicroRNA profiles transition from normal breast to ductal carcinoma in situ and transition to invasive ductal carcinoma (IDC) and methods of use thereof are described. Methods of diagnosis and prognosis using microRNA signatures to differentiate invasive from in situ carcinoma are described. Also described is the use of microRNA expression for predicting overall survival and time to metastasis.

主权项

1. A method of diagnosing whether a subject has breast invasive ductal carcinoma (IDC) as compared to ductal carcinoma in situ (DCIS), comprising:
a) reverse transcribing RNA from a test sample obtained from the subject to provide a set of target oligodeoxynucleotides wherein the subject has breast IDC; b) hybridizing the target oligodeoxynucleotides to a microarray comprising miR-210, let-7d and miR-221 specific probe oligonucleotides to provide a hybridization profile for the test sample; and c) comparing the test sample hybridization profile to a hybridization profile generated from a control sample, wherein an increase in the expression of miR-210, let-7d and miR-221 is indicative of the subject having IDC as compared to ductal carcinoma in situ (DCIS).