发明名称 |
GLYCOLYSIS-INHIBITING SUBSTANCES IN CELL CULTURE |
摘要 |
An improved system for large scale production of proteins and/or polypeptides in cell culture is provided. In accordance with the present invention, cells expressing the protein or polypeptide of interest are grown in media that comprise a glycolysis-inhibiting substance. Additionally and/or alternatively, cells expressing the protein or polypeptide of interest are grown in media in which glutamine is limited. The use of such a system allows high levels of protein or polypeptide production and lessens accumulation of undesirable metabolic waste products such as lactate. Proteins and polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical, immunogenic, agricultural or other commercial compositions. |
申请公布号 |
WO2008055260(A3) |
申请公布日期 |
2009.01.08 |
申请号 |
WO2007US83473 |
申请日期 |
2007.11.02 |
申请人 |
WYETH;WANG, WENGE;LUAN, YEN-TUNG |
发明人 |
WANG, WENGE;LUAN, YEN-TUNG |
分类号 |
C12N5/00;C12P21/00 |
主分类号 |
C12N5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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