Fermentative glycerol-free ethanol production

摘要

The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.

主权项

1. A transgenic yeast cell comprising one or more recombinant heterologous, nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10),
wherein said cell lacks enzymatic activity needed for the NADH-dependent glycerol synthesis, or said cell has a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to a corresponding wild-type yeast cell; and wherein at least one said NAD+-dependent acetylating acetaldehyde dehydrogenase is represented by SEQ ID NO:2 or by SEQ ID:29 or a functional homolog of SEQ ID NO:2 or SEQ ID NO:29, wherein said functional homolog has a sequence identity of at least 90% with SEQ ID NO:2 or SEQ ID NO:29, wherein said cell comprises one or more nucleic acid sequences encoding an acetyl-Coenzyme A synthetase activity (EC 6.2.1.1) and one or more nucleic acid sequences encoding NAD+-dependent alcohol dehydrogenase activity (EC 1.1.1.1).