| 摘要 |
This invention discloses a single capture affinity assay and apparatus for the identification and qualification of an analyte in a biological sample. The assay uses an array of affinity capture agents specific for an analyte wherein the analyte is removed prior to detection. After the analyte is bound to the capture agent, a moiety of the complex is chemically derivatized, then the analyte is dissociated from the capture agent, revealing underivatized moiety located in the analyted-capture agent contact interface, which is detected by binding with a signal tag and the signal is quantitated. Analyte detection is achieved without using labeled secondary detection agents. The method is especially applicable to proteins, with antibodies as capture agents and selected amino acids as lysine being derivatized, The method may be used for uniplex or multiplex or multiplexed analysis, and is applicable to high-throughput protien measurement. |
