Method of Excising Sugar Chain from Glycoprotein, Method of Mass Spectrometry of Sugar Chain, and Method of Mass Spectrometry of Glycoprotein

摘要

The present invention provides a method of enzymatic reaction on a membrane by a sugar chain releasing enzyme, for an MALDI-TOF MS analysis of a glycoprotein that is solid-phased on a membrane is conducted directly on the membrane; a method of mass spectrometry of a sugar chain in which a sugar chain, for the MALDI-TOF MS analysis of a sugar chain excised from a glycoprotein that is solid-phased on a membrane is conducted directly on the membrane; and a method of mass spectrometry of a glycoprotein, for the MALDI-TOF MS analysis of a glycoprotein of a glycoprotein that is solid-phased on a membrane is conducted directly on the membrane. A method of excising a sugar chain to obtain excised sugar chains by excising the sugar chains by dispensing a sugar chain releasing enzyme solution on a glycoprotein that is solid-phased on a carrier, wherein the sugar chain releasing enzyme solution is a solution containing a sugar chain releasing enzyme in a reaction buffer solution containing a buffering agent consisting essentially of a volatile component. A method of mass spectrometry of a sugar chain and a method of mass spectrometry of a glycoprotein using the method of excising the sugar chain.