发明名称 |
METHOD OF PRODUCING A POPULATION OF CELLS |
摘要 |
A method is provided for producing an in vitro or ex vivo method of producing a population of lineage committed haematopoietic progenitor, or mature haematopoietic cells, other than cells of the neutrophil lineage, which method comprises the steps of. (a) providing a population of cells comprising haematopoietic progenitor cells; and (b) culturing the population of cells in an animal cell culture medium comprising one or more cytokines that differentiate said progenitor cells into lineage committed haematopoietic progenitor and/or mature haematopoietic cells, under conditions of low oxidative stress, the culture medium being agitated when the cells are at a cell density at which oxygen transfer via the surface of the culture medium is insufficient for growth of the progenitor cells and the progeny thereof under static conditions, to produce a population of lineage committed haematopoietic progenitor, or mature haematopoietic cells, other than cells of the neutrophil lineage. The resulting population of cells can be used to increase the number of haematopoietic cells in a patient. |
申请公布号 |
CA2711549(C) |
申请公布日期 |
2016.08.23 |
申请号 |
CA20092711549 |
申请日期 |
2009.01.08 |
申请人 |
THE UNIVERSITY OF QUEENSLAND |
发明人 |
TIMMINS , NICHOLAS EION;NIELSEN, LARS KELD;PALFREYMAN, EMMA LOUISE |
分类号 |
C12N5/0789;A61K35/15;A61K35/17;A61K35/18;A61K35/19;A61K35/28;C12N5/078 |
主分类号 |
C12N5/0789 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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