ENHANCED CELL REPROGRAMMING BY MRNA

摘要

Described is a method of reprogramming a somatic cell into a reprogrammed induced neural stem/precursor cell (iNP cell), said method comprising the steps of: a) introducing one or more mRNAs encoding a transcription factor into said somatic cell; b) culturing said somatic cell under conditions permissive to the culture of said iNP cell. Moreover, there is also described a reprogrammed induced neural stem/precursor cell (iNP cell) produced by a method as described. Further, described is an mRNA encoding a transcription factor wherein said transcription factor is Sox2, Pax6 or Lmx1a and wherein said mRNA contains a combination of unmodified and modified nucleotides, wherein 5 to 50% of the uridine nucleotides and 5 to 50% of the cytidine nucleotides are respectively modified uridine nucleotides and modified cytidine nucleotides.