发明名称 |
METHODS OF DELIVERY OF MOLECULES TO CELLS USING A RICIN SUBUNIT AND COMPOSITIONS RELATING TO SAME |
摘要 |
A method of preparing molecules of interest for delivery to eukaryotic cells is shown, wherein a ricin B chain subunit not having a ribosome inactivating subunit and retaining lectin activity is modified by modifying the first cysteine residue to be absent or substituted with an amino acid other than cysteine, or removing a protease sensitive site at the N-terminal of the subunit, or adding an endoplasmic reticulum retrieval signal, and operatively associating the subunit with a molecule of interest. Methods of operatively associating the subunit and molecule of interest include chemical conjugation at primary amines, conjugation with N-glycans of the subunit, a disulfide bond, and assembly of immunoglobulin domains. The invention provides for operatively associating multiple molecules of interest with a ricin B chain subunit, and delivery into targeted cells, cell components, and combination of cells. |
申请公布号 |
WO2008157263(A3) |
申请公布日期 |
2009.03.05 |
申请号 |
WO2008US66790 |
申请日期 |
2008.06.12 |
申请人 |
ARKANSAS STATE UNIVERSITY;CRAMER, CAROL;REIDY, MICHAEL;DOLAN, MAUREEN |
发明人 |
CRAMER, CAROL;REIDY, MICHAEL;DOLAN, MAUREEN |
分类号 |
C07K14/415;A61K36/47;A61K39/395;C07K16/16 |
主分类号 |
C07K14/415 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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