NEW MONOCLONAL ANTIBODIES AGAINST THE RECEPTOR DEC-205 OF CHICKEN DENTRITIC CELLS

摘要

The invention relates to the production and the characterisation of new murine monoclonal antibodies that recognize the domain CTD-2 (SEQ ID NO: 1) of the cell receptor DEC-205 of dendritic cells in chickens (Gallus gallus), pigs, (Sus scrofa) and humans (Homo sapiens). The invention also relates to the capacity of the new antibodies to direct and modulate the immune response at different levels in chickens (Gallus gallus) and pigs (Sus scrofa), as well as recognising the receptor DEC-205 in dentritic cells and cell lines in humans, In addition, the invention is used to quickly produce a specific humoral immune response against Hemaglutinina H5 of the H5N2-type avian flu virus.

主权项

1. An antibody that recognizes the CTLD-2 domain of the DEC-205 receptor of Gallus gallus (SEQ ID NO. 1), characterized by the fact that it comprises a fragment VH with an amino acid sequence selected from the group that consists of SEQ ID NO. 15, SEQ ID NO. 16, and functionally equivalent variants in positions 26-35, 50-65, and 99-103 of said SEQ ID NO. 15 or SEQ ID NO. 16 and a fragment VL with a sequence selected from the group consisting of SEQ ID NO. 13 and SEQ ID NO. 14, and functionally equivalent variants in positions 24-40, 56-62, or 95-102 of said SEQ ID NO. 13 or SEQ ID NO. 14.