| 摘要 |
PROBLEM TO BE SOLVED: To provide methods for manufacturing mammalian glial restricted progenitor (GRP) cells, methods for decreasing unintended cellular phenotypes in a GRP cell population and/or methods for decreasing standard deviation in cells of a GRP cell population, as well as an antibody panel to characterize GRP cells, a method for its use in characterizing the cells as GRP cells, and gene expression profiles.SOLUTION: Provided is a method for manufacturing mammalian glial restricted progenitor (GRP) cells of a mammal lacking A2B5 positive cells, the method comprising: (a) isolating A2B5 antibody-reactive cells from a mammalian tissue source capable of generating A2B5 positive cells; (b) culturing the A2B5 positive cells for greater than 6 days in vitro (DIV) on a substrate; and (c) harvesting the cultured cells. Also provided are methods for use of the GRP cells to generate astrocytes and/or oligodendrocytes.SELECTED DRAWING: None |
