PROCESS AND REAGENT FOR OLIGONUCLEOTIDE SYNTHESIS AND PURIFICATION

摘要

PROBLEM TO BE SOLVED: To provide an improved synthesis process, a process usable for purification and a reaction reagent, for efficiently performing oligonucleotide synthesis.

SOLUTION: Compounds that scavenge acrylonitrile produced during the deprotection of phosphate groups bearing ethylnitrile protecting groups, are provided. In a preferred embodiment, the acrylonitrile scavenger is a polymer-bound thiol. In another aspect, the reaction agent used to oxidize a phosphite to a phosphate is provided. In a preferred embodiment, the oxidizing agent is sodium chlorite, chloroamine or pyridine-N-oxide.

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