| 摘要 |
Disclosed is a method of producing a virus like particle (VLP) in a plant comprising, a) introducing a first nucleic acid comprising a first regulatory region active in the plant and operatively linked to a nucleotide sequence encoding an influenza hemagglutinin (HA) protein selected from the group consisting of B and H3 into the plant, or portion of the plant, b) introducing a second nucleic acid comprising a second regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a proton channel protein c) incubating the plant or portion of the plant under conditions that permit the expression of the nucleic acids, thereby producing the VLP. Also disclosed is a method of producing a virus like particle (VLP) in a plant comprising, a) introducing a first nucleic acid comprising a first regulatory region active in the plant and operatively linked to a nucleotide sequence encoding influenza hemagglutinin (HA) protein B into the plant, or portion of the plant, wherein one or more proteolytic loop of the influenza HA protein has been deleted, b) incubating the plant or portion of the plant under conditions that permit the expression of the nucleic acids, thereby producing the VLP. |
