| 摘要 |
Dihydrocoumarin produced technologically from coumarin by biotransformation is claimed together with related production variants that are carried out with the aid of isolated enzymes and/or microorganisms. Pure coumarin as well as coumarin isolated from a plant extract or coumarin-containing plant extracts can be used as starting materials. Selected strains of Saccharomyces, Arthrobacter, Pseudomonas, Bacillus, Basidiomycetes and Fusarium can be directly used for the biotransformation or can provide the enzymes required for the biotransformation. Preferred enzymes are coumarate and coumarin reductases. A production variant is additionally claimed which starts with coumarin and which, via o-coumaric acid or via intermediary dihydrocoumarin, leads to the subsequent melilotic acid which is subsequently dehydrated to dihydrocoumarin. The dihydrocoumarin obtained in this manner fulfils all criteria of a natural raw material which is why the product can also be used as a natural flavouring or for the production of or as a component of natural, nature-identical and synthetic flavours as they are typically used in baked goods, sweets, beverages, crèmes, cereal products and milk products. |
