PRODUCTION OF 3-HYDROXYCARBOXYLIC ACID USING NITRILASE MUTANTS
摘要
This invention relates to nitrilase mutants having improved nitrilase activity for converting 3-hydroxynitriles to 3-hydroxycarboxylic acids. More specifically, the Acidavorax facilis 72W (ATCC 55746) nitrilase gene was mutated using error-prone PCR and site-directed mutagenesis to create nitrilase enzymes having improved nitrilase activity for converting 3-hydroxynitriles (e.g., 3-hydroxybutyronitrile or 3-hydroxyvaleronitrile) to the corresponding 3-hydroxycarboxylic acids. A process using these improved mutants to produce the 3-hydroxycarboxylic acids is also provided.
申请公布号
EP1778840(A2)
申请公布日期
2007.05.02
申请号
EP20050787796
申请日期
2005.08.16
申请人
E.I. DU PONT DE NEMOURS AND COMPANY
发明人
PAYNE, MARK, S.;DICOSIMO, ROBERT;O'KEEFE, DANIEL, P.