发明名称 |
ANTISENSE OLIGONUCLEOTIDE INHIBITING ABERRANT SPLICING AND METHOD OF USING THE SAME |
摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method of inhibiting aberrant splicing in a pre-mRNA molecule by using antisense oligonucleotides, to provide a method of upregulating gene expression, and to provide antisense oligonucleotide useful for performing the method. <P>SOLUTION: A method of inhibiting aberrant splicing in a pre-mRNA molecule containing a mutation is disclosed. When a mutation is present in the pre-mRNA, the mutation causes the pre-mRNA to be spliced incorrectly and produce an aberrant mRNA or mRNA fragment different from the mRNA ordinarily encoded by the pre-mRNA. The method comprises hybridizing an antisense oligonucleotide to the pre-mRNA molecule to create a duplex chain molecule under conditions which permit splicing. The antisense oligonucleotide is one which does not activate ribonuclease H, and is selected to block a member of the aberrant set of splice elements created by the mutation so that the normal intron is removed by splicing and the first mRNA molecule encoding a native protein is produced. Oligonucleotides useful for carrying out the method are also disclosed. <P>COPYRIGHT: (C)2008,JPO&INPIT |
申请公布号 |
JP2008092960(A) |
申请公布日期 |
2008.04.24 |
申请号 |
JP20080000648 |
申请日期 |
2008.01.07 |
申请人 |
UNIV NORTH CAROLINA AT CHAPEL HILL:THE |
发明人 |
KOLE RYSZARD;DOMINSKI ZBIGNIEW |
分类号 |
C12N15/09;A61K31/70;A61K38/00;A61K48/00;C07H21/04;C12N5/10;C12N15/113;C12P21/02 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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