SYSTEM AND METHOD FOR DETECTION OF HIV DRUG RESISTANT VARIANTS

摘要

In one embodiment of the invention a method for detecting low frequency o ccurrence of one or more HIV sequence variants associated with drug resistan ce is describe that comprises generating cDNA species from RNA molecules in an HIV sample population; amplifying first amplicons from the cDNA species, wherein each amplicon comprises amplified copies and is amplified with a pai r of nucleic acid primers that define a locus; clonally amplifying the ampli fied copies of the first amplicons to produce second amplicons that comprise an immobilized population of substantially identical copies from one of the amplified copies of first amplicons; determining a nucleic acid sequence co mposition from at least 100 of the immobilized populations in parallel on a single instrument; detecting one or more sequence variants that occur at a f requency of 5% or less in the nucleic acid sequence composition of the at le ast 100 immobilized populations; and correlating the detected sequence varia nts with variation associated with HIV drug resistance.