Methods for low cell-density bacterial protein expression that can achieve levels of up to 180 mg/l using a simple and low cost strategy. Full codon optimization is unnecessary to improve expression of viral genes rich in E. coli rare codons. Using a strategically modified host cell provides a simpler and cheaper alternative.
申请公布号
WO2009029665(A2)
申请公布日期
2009.03.05
申请号
WO2008US74483
申请日期
2008.08.27
申请人
THE REGENTS OF THE UNIVERSITY OF COLORADO;GARCEA, ROBERT, L.;MIDDELBERG, ANTON;CHUAN, YAP, PANG
发明人
GARCEA, ROBERT, L.;MIDDELBERG, ANTON;CHUAN, YAP, PANG