BLOOD DERIVED IMMUNE STIMULATORY COMPOSITIONS

摘要

Neutrophil extracellular traps (NETS) are webs of DNA held together with immunogenic peptides, released by neutrophils subsequent to activation. NETS are the most potent stimulator of dendritic cells, monocytes and T cells given their ability to activate TLR3, TLR4, TLR7 and TLR9. The use of NETS for in vivo stimulation of immunity in a therapeutic sense has not been utilized due to fear of anti-DNA antibody formation and subsequent development of systemic lupus erythromatosis. The current invention provides means of safely generating NETS in vitro through peripheral blood utilizing clinically safe means such as yeast-derived component zymosan, isolating said NETS, utilizing said NETS to in vitro activate cytokine production from PBMC in vitro, and concentrating said cytokines. Cytokines generated by this methodology have superior ability to stimulate NK cells in vitro as compared to isolated NK stimulatory cytokines. The invention provides means of utilizing said “symphony of cytokines” to treat cancer and viral infections.

主权项

1. A composition for the stimulation of immune responses, said composition derived by the steps of:
a) extracting a population of blood cells containing neutrophils; b) contacting said population of blood cells containing neutrophils with an agent capable of stimulating neutrophil extracellular trap formation; c) isolating said neutrophil extracellular traps; d) contacting said neutrophil extracellular traps with one or more immune cells for a time period sufficient to induce activation of said immune cells; e) collecting culture supernatant from said activated immune cells; and f) concentrating said culture supernatant.