| 发明名称 |
PLURIPOTENT STEM CELL EXPANSION AND PASSAGE USING A ROCKING PLATFORM BIOREACTOR |
| 摘要 |
Provided herein are novel methods for expansion and passaging of cell aggregates comprising stem cells and/or differentiated cells and comprising the use of closed systems on rocking platform bioreactors. The methods of the invention permit closed system serial passage expansion of pluripotent stem cells and/or progeny thereof with associated pluripotency markers and differentiation potential. |
| 申请公布号 |
US2016340633(A1) |
申请公布日期 |
2016.11.24 |
| 申请号 |
US201514956408 |
申请日期 |
2015.12.02 |
| 申请人 |
General Electric Company |
发明人 |
Davis Brian Michael;Conway Kenneth Roger;Loghin Evelina Roxana;Zhang Xiaohua |
| 分类号 |
C12M1/00;C12N5/074;C12N5/0735;C12M3/06;C12M1/36 |
主分类号 |
C12M1/00 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for expansion of cell aggregates in a closed system comprising
a cell culture vessel on a rocking platform bioreactor; aggregate formation in the vessel; automated perfusion of cell aggregates in the vessel; membrane-free filtration of cell aggregates during the perfusion; and aggregate harvest and passaging in the closed system. |
| 地址 |
Schenectady NY US |
