Gene Mutation Analyzer, Gene Mutation Analysis System, and Gene Mutation Analysis Method

摘要

An aspect of the present invention includes performing electrophoresis of a nucleic acid sample to be analyzed labeled for each base type, generating waveform data of detected intensity by detecting a label signal for the each base type, selecting another peak position for each peak position of the waveform data for each base type, calculating relative signal intensity of signal intensity in each position relative to the signal intensity in the other selected position, and analyzing existence of each base type in a base sequence coordinate position of the nucleic acid sample by comparing the relative signal intensity of the nucleic acid sample to be analyzed and the relative signal intensity of a known nucleic acid sample in each peak position. Accordingly, acquiring information about a gene mutation in trace amounts existing in a target gene region highly sensitively with high precision is realized.

主权项

1. A gene analyzer comprising:
a channel in which electrophoresis of a nucleic acid sample to be analyzed labeled for each base type is performed; a waveform data generator that generates waveform data of detected intensity of the nucleic acid sample the electrophoresis of which is performed in the channel by detecting a label signal for the each base type; a peak detector that detects a peak value of the waveform data for the each base type; a relative signal intensity calculation unit that, after at least one corresponding other peak position being selected under predetermined conditions for each peak position, calculates relative signal intensity of signal intensity in the each peak position relative to the signal intensity in the other selected peak position; and an analysis unit that generates information about existence of the each base type in a base sequence coordinate position of the nucleic acid sample to be analyzed by comparing the relative signal intensity of the nucleic acid sample to be analyzed and known relative signal intensity as the relative signal intensity of a known nucleic acid sample stored in advance in the each peak position.