发明名称 |
METHODS FOR STRUCTURAL DETERMINATION OF SELENIUM DERIVATIZED NUCLEIC ACID COMPLEXES |
摘要 |
Methods for crystallizing a molecule of interest, such as a polypeptide, in complex with nucleic acid, including contacting the molecule of interest with selenium-derivatized nucleic acid and crystallizing the molecule of interest/selenium-derivatized nucleic acid complex are provided. Methods for determining the X-ray crystal structure of molecule of interest/selenium-derivatized nucleic acid complexes are also provided. Typically, the method of X-ray crystal structural determination includes selenium single-wavelength anomalous phasing of the selenium-derivatized nucleic acid. In some embodiments the phases for the X-ray crystal structure of the molecule of interest are not provided from another crystal. Also disclosed are methods of affecting a biological process by administering a functional nucleic acid to a cell or a subject and/or by bringing into contact a nuclease and a functional nucleic acid, where the functional nucleic acid is selenium-derivatized nucleic acid. |
申请公布号 |
US2016279250(A1) |
申请公布日期 |
2016.09.29 |
申请号 |
US201415038242 |
申请日期 |
2014.11.21 |
申请人 |
SeNA Research ,Inc. |
发明人 |
HUANG Zhen |
分类号 |
A61K47/48;G06F19/00;C12N9/22;G01N33/53;A61K38/46;C12N9/96 |
主分类号 |
A61K47/48 |
代理机构 |
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代理人 |
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主权项 |
1. A method of crystallizing a molecule of interest in complex with nucleic acid, comprising the steps of
(a) contacting the molecule of interest with selenium-derivatized nucleic acid; and (b) crystallizing the molecule of interest/selenium-derivatized nucleic acid complex. |
地址 |
Marietta GA US |