Method of co-culturing mammalian muscle cells and motoneurons

摘要

The invention provides a method of co-culturing mammalian muscle cells and mammalian motoneurons. The method comprises preparing one or more carriers coated with a covalently bonded monolayer of trimethoxysilylpropyl diethylenetriamine (DETA); suspending isolated fetal mammalian skeletal muscle cells in serum-free medium according to medium composition 1; suspending isolated fetal mammalian spinal motoneurons in serum-free medium according to medium composition 1; plating the suspended muscle cells onto the one or more carriers at a predetermined density and allowing the muscle cells to attach; plating the suspended motoneurons at a predetermined density onto the one or more carriers and allowing the motoneurons to attach; covering the one or more carriers with a mixture of medium composition 1 and medium composition 2; and incubating the carriers covered in the media mixture.

主权项

1. A method of co-culturing mammalian muscle cells and motoneurons, the method comprising:
suspending fetal muscle cells and fetal spinal motoneurons in a serum-free medium according to composition 1 of Table 1; placing the suspended fetal muscle cells and fetal spinal motoneurons onto a monolayer of covalently bonded trimethoxysilylpropyl-diethylenetriamine supported on an underlying carrier surface; covering the carrier comprising muscle cells and motoneurons in a mixture of serum-free medium composition 1 of Table 1 and serum-free medium composition 2 of Table 2; and incubating the covered carrier comprising muscle cells and motoneurons.