发明名称 |
RECOMBINANT E. COLI EXPRESSING POLYHYDROXYALKANOATE BIOSYNTHETIC ENZYMES OF ALCALIGENES LATUS AND PROCESS FOR PRODUCING POLYHYDROXYALKANOATE USING IT |
摘要 |
PURPOSE: An economic process for producing high-concentrated polyhydroxyalkanoates, especially poly(3-hydroxybutylate)("P(3HB)") and poly(3-hydroxybutyrate-co-3-hydroxyvalerate)("P(3HB-co-3HV)") in a large scale is provided by using a recombinant E. coli, XL1-Blue-pJC3 (KCTC-0480BP) or XL1-Blue-pJC4 (KCTC-0481BP). CONSTITUTION: The recombinant E. coli, XL1-Blue-pJC3 is produced by transforming with pJC3 containing PHA operon and beta-lactamase gene of Alcaligenes latus. The recombinant E. coli, XL1-Blue-pJC4 is produced by transforming with pJC4 (figure 1c) containing PHA operon, beta-lactamase gene and plasmid R1 parB region of Alcaligenes latus. Polyhydroxyalkanoates are produced by culturing the E. coli XL1-Blue-pJC3 or XL1-Blue-pJC4 in a simple medium containing glucose as carbon source at 20-40°C.
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申请公布号 |
KR20000009279(A) |
申请公布日期 |
2000.02.15 |
申请号 |
KR19980029562 |
申请日期 |
1998.07.22 |
申请人 |
KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY |
发明人 |
LEE, SANG YEOP;CHOI, JONG IL |
分类号 |
C12N15/70;C12N15/60;(IPC1-7):C12N15/70 |
主分类号 |
C12N15/70 |
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