发明名称 |
ASSAY OF MICRO-ORGANISMS IN CELL CULTURES |
摘要 |
Micro-organisms which over-produce ATPase, especially mycoplasmas in a sample of a cell culture, are detected by making use of the ATPase activity to convert cellular or externally added ATP to ADP. The decrease in ATP externally added or the increase or absolute level of ADP produced from cellular ATP can then be detected or measured. For example, if a reagent such as phosphenol pyruvate/pyruvate kinase is added to convert that ADP back to ATP, the resulting high level of ATP can be detected bioluminescently using the reaction: Luciferin+luciferase enzyme+ATP->hv+products. |
申请公布号 |
EP1228239(B1) |
申请公布日期 |
2007.01.03 |
申请号 |
EP20000973075 |
申请日期 |
2000.11.08 |
申请人 |
LUMITECH (UK) LIMITED |
发明人 |
CROUCH, SHARON PATRICIA MARY;BRADBURY, DAWN ANN;SLATER, KEVIN JOHN |
分类号 |
C12Q1/04;G01N21/78;C12Q1/00;C12Q1/02;C12Q1/48;C12Q1/66;C12R1/35 |
主分类号 |
C12Q1/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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