NUCAMPHOLIN NUCLEIC ACID MOLECULES TO CONTROL COLEOPTERAN INSECT PESTS

摘要

This disclosure concerns nucleic acid molecules and methods of use thereof for control of insect pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in insect pests, including coleopteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of insect pests, and the plant cells and plants obtained thereby.

主权项

1. An isolated nucleic acid comprising at least one polynucleotide operably linked to a heterologous promoter, wherein the polynucleotide is selected from the group consisting of:
SEQ ID NO:1; the complement of SEQ ID NO:1; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:1; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:1; a native coding sequence of a Diabrotica organism comprising SEQ ID NO:1; the complement of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:1; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:1; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:1; SEQ ID NO:77; the complement of SEQ ID NO:77; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:77; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:77; a native coding sequence of a Diabrotica organism comprising SEQ ID NO:77; the complement of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:77; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:77; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:77; SEQ ID NO:84; the complement of SEQ ID NO:84; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:84; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:84; a native coding sequence of a Meligethes organism comprising SEQ ID NO:84; the complement of a native coding sequence of a Meligethes organism comprising SEQ ID NO:84; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Meligethes organism comprising SEQ ID NO:84; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Meligethes organism comprising SEQ ID NO:84; SEQ ID NO:86; the complement of SEQ ID NO:86; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:86; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:86; a native coding sequence of a Meligethes organism comprising SEQ ID NO:86; the complement of a native coding sequence of a Meligethes organism comprising SEQ ID NO:86; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Meligethes organism comprising SEQ ID NO:86; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Meligethes organism comprising SEQ ID NO:86; SEQ ID NO:88; the complement of SEQ ID NO:88; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:88; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:88; a native coding sequence of a Meligethes organism comprising SEQ ID NO:88; the complement of a native coding sequence of a Meligethes organism comprising SEQ ID NO:88; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Meligethes organism comprising SEQ ID NO:88; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Meligethes organism comprising SEQ ID NO:88; SEQ ID NO:93; the complement of SEQ ID NO:93; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:93; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:93; a native coding sequence of a Meligethes organism comprising SEQ ID NO:93; the complement of a native coding sequence of a Meligethes organism comprising SEQ ID NO:93; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Meligethes organism comprising SEQ ID NO:93; and the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Meligethes organism comprising SEQ ID NO:93.