IN SITU HEAT INDUCED ANTIGEN RECOVERY AND STAINING APPARATUS AND METHOD

摘要

Contemplated herein is an automated microscope slide antigen recovery and staining apparatus and method that features a plurality of individually operable miniaturized pressurizable reaction compartments for individually and independently processing a plurality of individual microscope slides. The apparatus preferably features independently movable slide support elements each having an individually heatable heating plate. Each slide support element may support a microscope slide. Each microscope slide can be enclosed within an individual pressurizable reaction compartment. Pressures exceeding 1 atm or below 1 atm can be created and maintained in the reaction compartment prior to, during or after heating of the slide begins. Because of the ability to pressurize and regulate pressure within the reaction compartment, and to individually heat each slide, each slide and a liquid solution or reagent thereon can be heated to temperatures that could not be obtained without the enclosed pressurized environment of the reaction compartment. A reagent dispensing strip having a plurality of reconfigurable reagent modules may also be used.

主权项

1. A method of treating a biological specimen, comprising:
disposing a microscope slide having the biological specimen thereon onto a slide support element of an automated apparatus, the apparatus comprising:
a plurality of reaction compartments, each having an inner space; anda plurality of slide support elements, wherein each slide support element is sized to support a microscope slide thereon, and wherein each slide support element is associated with one of the reaction compartments; positioning the biological specimen adjacent the inner space of the reaction compartment; causing the inner space of the reaction compartment to be sealed with a pressure-tight seal to form a sealed inner space containing the biological specimen; and pressurizing the sealed inner space of the reaction compartment to maintain an internal pressure above atmospheric pressure by heating a reagent or solution within the sealed inner space to a temperature exceeding 100° C.