METHOD FOR PRODUCING AN AMPEROMETRIC LACTATE BIOSENSOR, BIOSENSOR PRODUCED BY MEANS OF SAID METHOD, AND USE THEREOF IN TOXIC COMPLEX MEDIA

摘要

The invention relates to a method for producing an amperometric biosensor for the determination of lactate based on the lactate oxidase (LOD) enzyme, comprising the following steps: dispersion of a biopolymer in an organic solvent; preparation of a mediating solution by adding a mediator and carbon nanotubes; preparation of an enzymatic solution with at least the LOD enzyme; addition of the mediating and enzymatic solution to a disposable screen-printed platform; and drying of the biosensor. The invention also relates to the biosensor produced by means of said method, where the biopolymer is based on amylose and glucose polysaccharides with functional hydroxyl, amine, acetyl or carboxylic groups and has glycosidic bonds. The invention also relates to the use of same in complex media such as embryonic cell media, food media or blood samples.