Method of reducing pathogens in whole blood by illuminating with ultraviolet light under low oxygen conditions

摘要

Disclosed are methods for pathogen reduction in blood by illuminating with UV light while taking steps for preservation of blood components and reducing oxidative damage from reactive oxygen species generated during UV light exposure. Fresh whole donor blood or products derived therefrom may be submitted to UV light treatment while in a low oxygenation state defined by a partial oxygen pressure below a normal level. Such low oxygenation state occurs naturally shortly after blood collection or may be induced by exposure to low-oxygen environment, reducing temperature or by other disclosed steps.

主权项

1. A method of donor blood preservation and reducing pathogens by illuminating with ultraviolet light, the method comprising the steps of:
a) collecting fresh whole blood from a donor; b) providing the whole fresh blood in a low oxygenation state solely by performing step c) within 8 hours of collection, wherein a low oxygen state is defined by a partial oxygen pressure being below 160 mmHg; c) illuminating said whole blood with ultraviolet light at a wavelength range from 250 nm to 280 nm with intensity sufficient to reduce pathogens therein; and d) storing said whole blood for subsequent use with subjects other than said donor.