发明名称 |
SYNTHETIC SINGLE GUIDE RNA FOR CAS9-MEDIATED GENE EDITING |
摘要 |
The present invention provides synthetic single guide RNAs that comprise two separate functional sequences (commonly known as crRNA and tracrRNA) connected by a linker. These synthetic single guide RNA molecules are useful in gene editing when used with RNA-guided endonucleases such as cas9 in eukaryotic cells. The availability of the synthetic single guide RNAs makes the screening for gene editing in high-through-put format simple and convenient. |
申请公布号 |
WO2016186745(A1) |
申请公布日期 |
2016.11.24 |
申请号 |
WO2016US26444 |
申请日期 |
2016.04.07 |
申请人 |
GE HEALTHCARE DHARMACON, INC. |
发明人 |
HE, Kaizhang;ANDERSON, Emily Marie;DELANEY, Michael Oren |
分类号 |
C12N9/22;C12N15/09;C12N15/10 |
主分类号 |
C12N9/22 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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