PEPTIDE TOXIN FORMULATION

摘要

Procedures are described which use solvents to increase the topical insecticidal activity of toxic insect peptides. These procedures comprise drying the peptides, if needed, followed by the addition of either: 1) a polar organic solvent, with or without water, to a dried peptide, or 2) the addition of polar aprotic solvent or other adjuvant to the dried peptide, followed by the addition of either: 1) a polar organic solvent, with or without water, (where a polar aprotic solvent is added first) or 2) a polar aprotic solvent or other adjuvant to the peptide polar organic solvent (where the polar organic solvent is added first), to the peptide formulation.

主权项

1. A process for increasing the topical insecticidal activity of a peptide that is toxic to insects comprising making a topical toxic peptide formulation comprised of the following three components mixed together; 1) a peptide that is toxic to insects with 2) a polar aprotic solvent and 3) a polar organic solvent according to the following procedure:
a) selecting a peptide that is toxic to insects and that is less than about 200 amino acids and greater than about 10 amino acids; and b) mixing a first type of solvent with said peptide, wherein said first type of solvent is either a polar aprotic solvent or a polar organic solvent, to make a first solvent peptide mixture; c) mixing a second type of solvent with said first solvent peptide mixture, wherein said second type of solvent is not the same type of solvent as said first type of solvent and is either a polar aprotic solvent or a polar organic solvent, to make a topical toxic peptide formulation;wherein said polar aprotic solvent is selected from: dimethyl sulfoxide (DMSO)., dimethylformamide, dioxane and hexamethylphosphorotriamide andwherein said polar organic solvent is selected from propanol and all its isomers, methyl ethyl ketone, diethyl ketone, acetonitrile, and ethyl acetoacetate saving said topical toxic peptide formulation.