| 摘要 |
In this method for detecting a genetic mutation, real-time PCR is performed using: template DNA that has a double-stranded DNA containing a base sequence that is identical to a genomic region containing a target genetic mutation site; a forward primer and a reverse primer that are configured to amplify, via PCR, the region containing the genetic mutation site; and a wild-type oligonucleotide that contains at least one unnatural nucleic acid and has a base sequence that is complementary to a genomic region in which the genetic mutation site is wild-type. A double-stranded DNA in which the genetic mutation site is mutant-type is detected amid the template DNA on the basis of the total amount of PCR-amplified products that were obtained or on the basis of the amount of nucleic acids, among the PCR-amplified products, at which the genetic mutation site is mutant-type. The unnatural nucleic acid is an LNA or a BNA. In a first single-stranded DNA from among two single-stranded DNA that constitute the template DNA, said first single-stranded DNA hybridizing with the wild-type oligonucleotide, the region with which the wild-type oligonucleotide hybridizes and the region with which the forward primer or the reverse primer hybridizes are separated by 1-18 bases, or partially overlap. |
