发明名称 |
USE OF PERFUSION SEED CULTURES TO IMPROVE BIOPHARMACEUTICAL FED-BATCH PRODUCTION CAPACITY AND PRODUCT QUALITY |
摘要 |
Methods of improving the efficiency of production of a protein product of interest in mammalian cell culture are presented. In particular, the methods result in an increase in the quantity of a protein product produced, or decreases protein product production time in a manufacturing-scale bioreactor cell culture. The disclosed methods comprise: (a) culturing the N-1 bioreactor culture to high viable cell densities; and (b) seeding the production bioreactor culture at high viable cell seeding densities. |
申请公布号 |
EP3083933(A1) |
申请公布日期 |
2016.10.26 |
申请号 |
EP20140827357 |
申请日期 |
2014.12.19 |
申请人 |
BIOGEN MA INC. |
发明人 |
YANG, WILLIAM C.;LU, JIUYI;HUANG, YAO-MING;YUAN, HANG;KSHIRSAGAR, RASHMI;RYLL, THOMAS |
分类号 |
C12M1/34 |
主分类号 |
C12M1/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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