| 摘要 |
FIELD: medicine. ^ SUBSTANCE: invention refers to biotechnology and virology. Method of virus replication provides cultivation of permissive cells on an insoluble porous carrier, further infection thereof with virus to be replicated in the cells attached to the carrier. Said permissive cells are those of human or animal. The virus replicable in such cells functions as an infecting, and the insoluble porous carrier is polymer cryogel with connected pores of section 50-500 mcm. As a result, offered invention implemented enables the effect of eliminated undesired clogging of the carrier pores with the growing culture and cell debris, ensures greater universality of the method with respect to permissive cell types. Herewith more effective use of the whole carrier and culture medium with reduced risk of failed sterility and cost price of an end product, higher yield of the end virus. ^ EFFECT: invention can be used in applied virology, manufacturing of vaccines, nanobiotechnology, molecular biology and other fields of science and technique where production of preparative volumes of a viral material is required. ^ 3 cl, 1 tbl, 1 ex |
