| 发明名称 |
PRODUCTION OF GLYCOPROTEINS USING MANGANESE |
| 摘要 |
Culture media comprising manganese and methods of culturing cells to improve sialylation and glycosylation of glycoproteins are provided. |
| 申请公布号 |
US2016222075(A1) |
申请公布日期 |
2016.08.04 |
| 申请号 |
US201615004716 |
申请日期 |
2016.01.22 |
| 申请人 |
Amgen Inc. |
发明人 |
Crowell Christopher Kenyon;Grampp Gustavo Enrique |
| 分类号 |
C07K14/505 |
主分类号 |
C07K14/505 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for producing an erythropoietic composition comprising sialylated erythropoiesis-stimulating molecules, comprising the steps of:
growing a manganese-responsive host cell in a culture medium containing an amount of manganese effective to increase the sialylation of said erythropoietic composition, wherein the concentration of manganese in said culture medium ranges from about 0.01 to about 40 μM. |
| 地址 |
Thousand Oaks CA US |
