FUSION TAGS FOR PROTEIN EXPRESSION

摘要

Fluorescent and non-fluorescent fusion tags derived from green fluorescent protein (GFP). The fusion tags harbor substitutions with respect to GFP that increase expression of target proteins to which the fusion tags are fused; increase solubility; prevent dimerization and oligomerization, particularly in oxidizing environments such as the endoplasmic reticulum (ER); and, in some cases, enhance fluorescence of the fusion tag itself. The substitutions include various combinations of substitutions at select cysteine residues in GFP, substitutions in or near the GFP chromophore, and/or other substitutions. The fusion tags can be used for increasing expression of target proteins for mass production thereof or as a fluorescent tag.

主权项

1. A polynucleotide comprising a fusion tag-encoding sequence, the fusion tag-encoding sequence encoding a polypeptide sequence at least about 80% identical to SEQ ID NO:2, wherein the fusion tag-encoding sequence encodes a residue other than cysteine at a position corresponding to position 49 of SEQ ID NO:2 and a residue other than cysteine at a position corresponding to position 71 of SEQ ID NO:2.