Disclosed are methods and procedures which may be applied to cells (including differentiating (or partially differentiated) cells, cells in the terminal stages of differentiation and/or differentiated cells) to facilitate late stage maturation towards mature erythrocyte lineages – in particular towards reticulocytes and/or enucleated (anucleate) mature erythrocytes. The disclosure provides a method in which mature enucleated erythrocytes and/or reticulocytes are produced maintaining and/or culturing stem cells (for example differentiating or differentiated stem cells) under one or more maturation conditions in which the pH, level of (dissolved) oxygen; and level of mechanical stress is controlled.
申请公布号
WO2016116769(A1)
申请公布日期
2016.07.28
申请号
WO2016GB50146
申请日期
2016.01.22
申请人
SCOTTISH NATIONAL BLOOD TRANSFUSION SERVICE;UNIVERSITY COURT OF THE UNIVERSITY OF GLASGOW;LOUGHBOROUGH UNIVERSITY INNOVATIONS LIMITED