Delta-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts

摘要

The present invention relates to a Delta12 fatty acid desaturase able to catalyze the conversion of oleic acid to linoleic acid (LA; 18:2). Nucleic acid sequences encoding the desaturase, nucleic acid sequences that hybridize thereto, DNA constructs comprising the desaturase gene, and recombinant host microorganisms expressing increased levels of the desaturase are described. Methods of increasing production of specific omega-3 and/or omega-6 fatty acids are described by overexpression of the Delta12 fatty acid desaturase or by disruption of the native gene.