CULTURE MEDIUM FOR ESTABLISHING NEUROEPITHELIAL STEM CELLS, METHOD FOR ESTABLISHING NEUROEPITHELIAL STEM CELLS, AND APPLICATIONS THEREOF

摘要

The present invention discloses a culture medium for establishing neuroepithelial stem cells, a method for establishing neuroepithelial stem cells, and the applications thereof, wherein the culture medium comprises differentiation culture medium for inducing and differentiating pluripotent stem cells to elementary neuroepithelial stem cells as well as proliferation culture medium for amplifying the elementary neuroepithelial stem cells; and the pluripotent stem cells can be differentiated to the elementary neuroepithelial stem cells in the differentiation culture medium. While the elementary neuroepithelial stem cells have the functions of the neuroepithelial stem cells, they cannot be stably amplified in long term culture under the conventional culture conditions, however they are maintained the stability properties in long term culture in the proliferation culture medium. The proliferation culture medium can culture the elementary neuroepithelial stem cells to the neuroepithelial stem cells, which are maintained stable amplification and survival, and can be produced in large scale.

主权项

1. A culture medium useful in either inducing differentiation of a pluripotent stem cells to an elementary neuroepithelial cell or proliferation of said elementary neuroepithelial cell, comprising:
(i) a neurobasal culture medium; (ii) a B-27 supplement; (iii) an N-2 supplement; (iv) bFGF; (v) an agonist of a Wnt signaling pathway; (vi) a GSK inhibitor; (vii) an inhibitor of a TGF-β signaling pathway, and (viii) either both an inhibitor of a Notch signaling pathway and an inhibitor of an ALK2/ALK3 signaling pathway, or leukemia inhibitory factor.