ASSAY FOR PRIONS

摘要

The invention relates to a method for detection of abnormal PrP in a sample of blood or urine, said method comprising:
(a) diluting the sample with buffer to comprise final concentrations of
(i) 10 mM to 500 mM buffer agent;(ii) 1% to 10% w/v bovine serum albumin; and(iii) 1% to 8% w/v CHAPS;(b) adding steel particles and incubating to allow PrP binding;(c) washing the steel particles to remove diluted sample; and(d) detecting abnormal PrP captured on the steel particles using antibody capable of binding said abnormal PrP.;The invention also provides compositions and kits.

主权项

1. A method for detection of abnormal PrP in a sample of urine, said method comprising:
(a) diluting the urine sample with buffer to comprise final concentrations of
(i) 10 mM to 500 mM buffer agent;(ii) 1% to 10% w/v bovine serum albumin; and(iii) 1% to 8% w/v 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS);wherein said buffer does not include chaotropic agents or proteinases; (b) adding steel particles and incubating to allow PrP binding; (c) washing the steel particles to remove diluted sample; (d) subjecting the washed steel particles to a heat treatment; and (e) detecting abnormal PrP captured on the steel particles using antibody or portion thereof capable of binding said abnormal PrP; wherein said method is practiced without use of chaotropic agents or proteinases.